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A Mutation That Improves Soluble Recombinant Hemoglobin Accumulation in Escherichia coli in Heme Excess

High-level expression of soluble recombinant human hemoglobin (rHb) in Escherichia coli was obtained with several hemoglobin variants. Under identical conditions, two rHbs containing the Presbyterian mutation (Asn-108→Lys) in β-globin accumulated to approximately twofold less soluble globin than rHb...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Appl Environ Microbiol
Päätekijät: Weickert, Michael J., Pagratis, Maria, Glascock, Christopher B., Blackmore, Richard
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology (ASM) 1999
Aiheet:
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91073/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9925594/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.2.640-647.1999
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