Fate of Free DNA and Transformation of the Oral Bacterium Streptococcus gordonii DL1 by Plasmid DNA in Human Saliva

Competitive PCR was used to monitor the survival of a 520-bp DNA target sequence from a recombinant plasmid, pVACMC1, after admixture of the plasmid with freshly sampled human saliva. The fraction of the target remaining amplifiable ranged from 40 to 65% after 10 min of exposure to saliva samples fr...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Mercer, Derry K., Scott, Karen P., Bruce-Johnson, Wendy A., Glover, L. Anne, Flint, Harry J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Environmental and Public Health Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90975/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9872752/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.1.6-10.1999
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