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Utility of Green Fluorescent Nucleic Acid Dyes and Aluminum Oxide Membrane Filters for Rapid Epifluorescence Enumeration of Soil and Sediment Bacteria

High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4′,6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circu...

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Publicado en:Appl Environ Microbiol
Autores principales: Weinbauer, Markus G., Beckmann, Christiane, Höfle, Manfred G.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 1998
Materias:
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90955/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9835595/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.12.5000-5003.1998
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