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Utility of Green Fluorescent Nucleic Acid Dyes and Aluminum Oxide Membrane Filters for Rapid Epifluorescence Enumeration of Soil and Sediment Bacteria

High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4′,6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circu...

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Bibliografische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Weinbauer, Markus G., Beckmann, Christiane, Höfle, Manfred G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1998
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90955/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9835595/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.12.5000-5003.1998
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