Insertion-Duplication Mutagenesis in Streptococcus pneumoniae: Targeting Fragment Length Is a Critical Parameter in Use as a Random Insertion Tool

To examine whether insertion-duplication mutagenesis with chimeric DNA as a transformation donor could be valuable as a gene knockout tool for genomic analysis in Streptococcus pneumoniae, we studied the transformation efficiency and targeting specificity of the process by using a nonreplicative vec...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Lee, Myeong S., Seok, Chaok, Morrison, Donald A.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90924/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9835564/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.12.4796-4802.1998
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