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Discrimination of SHV β-Lactamase Genes by Restriction Site Insertion-PCR

Restriction site insertion-PCR (RSI-PCR) is a simple, rapid technique for detection of point mutations. This technique exploits primers with one to three base mismatches near the 3′ end to modulate a restriction site. We have developed this technique to identify described mutations of the bla(SHV) g...

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Detalhes bibliográficos
Publicado no:Antimicrob Agents Chemother
Principais autores: Chanawong, Aroonwadee, M'Zali, Fatima Hannachi, Heritage, John, Lulitanond, Aroonlug, Hawkey, Peter Michael
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90608/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11408231/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AAC.45.7.2110-2114.2001
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