Discrimination of SHV β-Lactamase Genes by Restriction Site Insertion-PCR

Restriction site insertion-PCR (RSI-PCR) is a simple, rapid technique for detection of point mutations. This technique exploits primers with one to three base mismatches near the 3′ end to modulate a restriction site. We have developed this technique to identify described mutations of the bla(SHV) g...

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Bibliographische Detailangaben
Hauptverfasser: Chanawong, Aroonwadee, M'Zali, Fatima Hannachi, Heritage, John, Lulitanond, Aroonlug, Hawkey, Peter Michael
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2001
Schlagworte:
Mechanisms of Resistance
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC90608/
https://ncbi.nlm.nih.gov/pubmed/11408231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.7.2110-2114.2001
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