Molecular and Biochemical Analysis of AST-1, a Class A β-Lactamase from Nocardia asteroides Sensu Stricto

A β-lactamase gene was cloned from a Nocardia asteroides sensu stricto clinical isolate. A recombinant plasmid, pAST-1, expressed the β-lactamase AST-1 in Escherichia coli JM109. Its pI was 4.8, and its relative molecular mass was 31 kDa. E. coli JM109(pAST-1) was resistant to penicillins and narrow...

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Bibliographic Details

Published in:	Antimicrob Agents Chemother
Main Authors:	Poirel, Laurent, Laurent, Frederic, Naas, Thierry, Labia, Roger, Boiron, Patrick, Nordmann, Patrice
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 2001
Subjects:	Mechanisms of Resistance
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90387/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11181374/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AAC.45.3.878-882.2001
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