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Novel Scintillation Proximity Assay for Measuring Membrane-Associated Steps of Peptidoglycan Biosynthesis in Escherichia coli

We have developed a novel, high-throughput scintillation proximity assay to measure the membrane-associated steps (stages 2 and 3) of peptidoglycan synthesis in Escherichia coli. At least five enzymes are involved in these two stages, all of which are thought to be essential for the survival of the...

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Autors principals: Chandrakala, B., Elias, Bertha C., Mehra, Upasana, Umapathy, N. S., Dwarakanath, P., Balganesh, T. S., deSousa, Sunita M.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC90371/
https://ncbi.nlm.nih.gov/pubmed/11181358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.45.3.768-775.2001
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