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Flow Cytometric Monitoring of Antibiotic-Induced Injury in Escherichia coli Using Cell-Impermeant Fluorescent Probes

Three fluorescent nucleic acid binding dyes—propidium iodide, TO-PRO-1, and SYTOX green—were evaluated, and their abilities to distinguish between bacterial cells with and without an intact cytoplasmic membrane were compared. Each dye was readily able to discriminate between healthy and permeabilize...

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Udgivet i:Antimicrob Agents Chemother
Principais autores: Mortimer, Fiona C., Mason, David J., Gant, Vanya A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2000
Fag:
Mechanisms of Action: Physiological Effects
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC89745/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10681337/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.44.3.676-681.2000
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