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Identification and Characterization of Immunoglobulin G in Blood as a Major Inhibitor of Diagnostic PCR

A major inhibitor of diagnostic PCR in human plasma was identified and the mechanism of inhibition was characterized. Human blood was divided by centrifugation into buffy coat, plasma, platelets, and erythrocytes. All these blood fractions were found to be highly inhibitory to a standardized PCR mix...

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Hlavní autoři: Al-Soud, Waleed Abu, Jönsson, Leif J., Rådström, Peter
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2000
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC88721/
https://ncbi.nlm.nih.gov/pubmed/10618113
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