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Optimized PCR Using Patient Blood Samples For Diagnosis and Follow-Up of Visceral Leishmaniasis, with Special Reference to AIDS Patients

We developed a highly sensitive PCR method that enables the diagnosis and posttherapeutic follow-up of visceral leishmaniasis with patient blood. The PCR assay was thoroughly optimized by successive procedural refinements to increase its sensitivity and specificity. It was compared to in vitro culti...

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Published in:J Clin Microbiol
Main Authors: Lachaud, Laurence, Dereure, Jacques, Chabbert, Elisabeth, Reynes, Jacques, Mauboussin, Jean-Marc, Oziol, Eric, Dedet, Jean-Pierre, Bastien, Patrick
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2000
Subjects:
Parasitology
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC88701/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10618093/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.1.236-240.2000
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