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Use of PCR and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Techniques for Differentiation of Prevotella intermedia Sensu Stricto and Prevotella nigrescens

Primers were designed from 16S rRNA sequences of Prevotella intermedia sensu stricto and Prevotella nigrescens and were used to discriminate these two species by PCR. The results were compared with those from the PCR technique using primers designed from arbitrarily primed PCR products by Guillot an...

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Detalhes bibliográficos
Main Authors: Premaraj, Thyagaseely, Kato, Naoki, Fukui, Katsuhito, Kato, Haru, Watanabe, Kunitomo
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC88649/
https://ncbi.nlm.nih.gov/pubmed/10074526
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