Quantification of Proviral Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR

We have developed and evaluated a new method to quantify human immunodeficiency virus type 2 (HIV-2) proviral DNA based on LightCycler real-time PCR. The assay has a detection limit of 5 copies/10(5) peripheral blood mononuclear cells (PBMC) and is insensitive to HIV-2 strain variability: HIV-2 subt...

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Bibliografiske detaljer
Main Authors: Damond, Florence, Descamps, Diane, Farfara, Isabelle, Telles, Jean Noël, Puyeo, Sophie, Campa, Pauline, Leprêtre, Annie, Matheron, Sophie, Brun-Vezinet, Françoise, Simon, François
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Virology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC88534/
https://ncbi.nlm.nih.gov/pubmed/11724830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.12.4264-4268.2001
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