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Use of Quantitative PCR To Measure Density of Borrelia burgdorferi in the Midgut and Salivary Glands of Feeding Tick Vectors

Quantitative real-time PCR was used to assay spirochetes in feeding ticks. Spirochetes in tick midguts increased sixfold, from 998 per tick before attachment to 5,884 at 48 h of attachment. Spirochetes in tick salivary glands increased >17-fold, from 1.2 per salivary gland pair before feeding to...

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Detalhes bibliográficos
Main Authors: Piesman, Joseph, Schneider, Bradley S., Zeidner, Nordin S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC88501/
https://ncbi.nlm.nih.gov/pubmed/11682544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.11.4145-4148.2001
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