Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

Dengue virus (DV) was detected early in infected mosquito C6/36 cells by using indirect immunofluorescence (IF) in conjunction with flow cytometry. Three fixation-permeabilization methods and three DV serotype 1 (DEN-1)-specific monoclonal antibodies, 8-8 (anti-E), 16-4 (anti-NS1), and 15F3-1 (anti-...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kao, Chuan-Liang, Wu, Meng-Chan, Chiu, Yen-Hui, Lin, Jing-Lin, Wu, Yin-Chang, Yueh, Yi-Yung, Chen, Li-Kuang, Shaio, Men-Fang, King, Chwan-Chuen
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2001
Schlagworte:
Virology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC88405/
https://ncbi.nlm.nih.gov/pubmed/11574589
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.10.3672-3677.2001
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