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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains

Multiplex PCR amplification followed by either agarose gel electrophoresis (PCR-AGE) or microchip electrophoresis (PCR-ME) was used to test a total of 120 fungal strains. The internal transcribed spacer 1 (ITS1) and ITS2 regions and the 5.8S ribosomal DNA (rDNA) region of the fungi were amplified by...

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Udgivet i:J Clin Microbiol
Principais autores: Fujita, Shin-Ichi, Senda, Yasuko, Nakaguchi, Shigeki, Hashimoto, Takuma
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Mycology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC88398/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11574582/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.10.3617-3622.2001
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