Detection of Rifampin-Resistant Mycobacterium tuberculosis in Sputa by Nested PCR-Linked Single-Strand Conformation Polymorphism and DNA Sequencing

Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis. However, due to the nonspecific amplification of...

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Bibliografische Detailangaben

Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Kim, Bum-Joon, Lee, Keun-Hwa, Park, Bo-Na, Kim, Seo-Jeong, Park, Eun-Mi, Park, Young-Gil, Bai, Gil-Han, Kim, Sang-Jae, Kook, Yoon-Hoh
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2001
Schlagworte:	Mycobacteriology and Aerobic Actinomycetes
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC88194/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11427578/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.7.2610-2617.2001
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