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Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-Positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens

The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and sample...

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Bibliographic Details
Main Authors: Van Dyck, E., Smet, H., Van Damme, L., Laga, M.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC88124/
https://ncbi.nlm.nih.gov/pubmed/11376070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.6.2280-2282.2001
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