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Endocarditis Caused by Culture-Negative Organisms Visible by Brown and Brenn Staining: Utility of PCR and DNA Sequencing for Diagnosis

Two cases of culture-negative endocarditis with cocci seen in valve vegetations are presented. The organisms were identified by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene, sequencing, and database search using BLAST software. The results and utility of this m...

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Published in:J Clin Microbiol
Main Authors: Wilck, Marissa B., Wu, Yanyun, Howe, John G., Crouch, Jill Y., Edberg, Stephen C.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2001
Subjects:
Case Reports
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC88076/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11326041/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.5.2025-2027.2001
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