Use of a LightCycler gyrA Mutation Assay for Rapid Identification of Mutations Conferring Decreased Susceptibility to Ciprofloxacin in Multiresistant Salmonella enterica Serotype Typhimurium DT104 Isolates

A LightCycler-based PCR-hybridization gyrA mutation assay (GAMA) was developed to rapidly detect gyrA point mutations in multiresistant (MR) Salmonella enterica serotype Typhimurium DT104 with decreased susceptibility to ciprofloxacin (MIC, 0.25 to 1.0 mg/liter). Ninety-two isolates (49 human, 43 an...

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Bibliographic Details
Main Authors: Walker, R. A., Saunders, N., Lawson, A. J., Lindsay, E. A., Dassama, M., Ward, L. R., Woodward, M. J., Davies, R. H., Liebana, E., Threlfall, E. J.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC87952/
https://ncbi.nlm.nih.gov/pubmed/11283069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.4.1443-1448.2001
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