Detection of Mycobacterium bovis in Bovine Clinical Specimens Using Real-Time Fluorescence and Fluorescence Resonance Energy Transfer Probe Rapid-Cycle PCR

Nucleic acid sequence capture extraction was coupled with LightCycler PCR amplification and product detection using real-time fluorescence for rapid, definitive detection of Mycobacterium bovis in lymph node specimens from 38 cattle with bovine tuberculosis lesions. PCR amplification of sequence-cap...

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Detalhes bibliográficos
Main Authors: Taylor, Malcolm James, Hughes, Mary Siobhan, Skuce, Robin Alfred, Neill, Sydney Donnelly
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Mycobacteriology and Aerobic Actinomycetes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC87923/
https://ncbi.nlm.nih.gov/pubmed/11283040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.4.1272-1278.2001
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