Identification of Canine Coronavirus Strains from Feces by S Gene Nested PCR and Molecular Characterization of a New Australian Isolate

A nested PCR (nPCR) assay for the detection of canine coronavirus (CCV) in fecal samples is described. The target sequence for the assay was a 514-bp fragment within the spike (S) glycoprotein gene. The sensitivity of the assay is extremely high, detecting as little as 25 50% tissue culture infectiv...

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Detalhes bibliográficos
Main Authors: Naylor, Matthew J., Harrison, Gavan A., Monckton, Robert P., McOrist, Steven, Lehrbach, Philip R., Deane, Elizabeth M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC87870/
https://ncbi.nlm.nih.gov/pubmed/11230424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.3.1036-1041.2001
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