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Comparison of Various Sample Preparation Methods for PCR Diagnosis of Visceral Leishmaniasis Using Peripheral Blood

We have compared various sample preparation methods for the PCR diagnosis of visceral leishmaniasis (VL) using peripheral blood samples and tested the influence of these protocols upon sensitivity. Four methods of lysis-DNA extraction were used with two types of blood samples: whole blood (WB) and b...

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Detalhes bibliográficos
Main Authors: Lachaud, Laurence, Chabbert, Elisabeth, Dubessay, Pascal, Reynes, Jacques, Lamothe, Jacques, Bastien, Patrick
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC87785/
https://ncbi.nlm.nih.gov/pubmed/11158116
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.2.613-617.2001
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