Binary Typing of Staphylococcus aureus Strains through Reversed Hybridization Using Digoxigenin-Universal Linkage System-Labeled Bacterial Genomic DNA

A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasib...

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Main Authors: van Leeuwen, Willem, Libregts, Christel, Schalk, Marjolijn, Veuskens, Jack, Verbrugh, Henri, van Belkum, Alex
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2001
Teme:
Bacteriology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC87723/
https://ncbi.nlm.nih.gov/pubmed/11136792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.328-331.2001
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