Simultaneous Detection and Typing of Influenza Viruses A and B by a Nested Reverse Transcription-PCR: Comparison to Virus Isolation and Antigen Detection by Immunofluorescence and Optical Immunoassay (FLU OIA)

A nested reverse transcription (RT)-PCR was developed for simultaneous detection and typing of influenza viruses A and B. The detection limit for influenza virus A subtypes H1 and H3 and that for influenza virus B were between 1 and 4 target gene copies per reaction for each type. The clinical benef...

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Detalhes bibliográficos
Main Authors: Herrmann, Björn, Larsson, Christine, Zweygberg, Benita Wirgart
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC87692/
https://ncbi.nlm.nih.gov/pubmed/11136761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.134-138.2001
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