Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales

An internal partial sequence of the gene encoding actin (ACT), 725 to 762 bp in length, was amplified by PCR from the genomic DNA extract of 12 species of dermatophytes and sequenced. An intron that is 56 to 93 bp in length was located along the ACT fragment of all of the dermatophytes at codon posi...

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Bibliographic Details
Main Authors:	Okeke, Charles N., Tsuboi, Ryoji, Kawai, Masaaki, Hiruma, Masataro, Ogawa, Hideoki
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology 2001
Subjects:	Mycology
Online Access:	https://ncbi.nlm.nih.gov/pmc/articles/PMC87687/ https://ncbi.nlm.nih.gov/pubmed/11136756 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.101-106.2001
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Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales

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