Rapid Detection of Smear-Negative Mycobacterium tuberculosis by PCR and Sequencing for Rifampin Resistance with DNA Extracted Directly from Slides

Conventional methods for identification of Mycobacterium tuberculosis from culture can take 6 weeks. To facilitate the rapid detection of M. tuberculosis and to assess the risks of drug resistance, we developed a technique of eluting DNA directly from sputum slides and performing PCR for the detecti...

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Detalles Bibliográficos
Autores principales: Patnaik, Madhumita, Liegmann, Karsten, Peter, James B.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2001
Materias:
Mycobacteriology and Aerobic Actinomycetes
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC87678/
https://ncbi.nlm.nih.gov/pubmed/11136747
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.1.51-52.2001
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