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Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue Specimens

A procedure based on panfungal PCR and multiplex liquid hybridization was developed for the detection of fungi in tissue specimens. The PCR amplified the fungal internal transcribed spacer (ITS) region (ITS1-5.8S rRNA-ITS2). After capture with specific probes, eight common fungal pathogens (Aspergil...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Hendolin, Panu H., Paulin, Lars, Koukila-Kähkölä, Pirkko, Anttila, Veli-Jukka, Malmberg, Henrik, Richardson, Malcolm, Ylikoski, Jukka
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2000
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87561/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11060088/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.11.4186-4192.2000
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