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Rapid Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi Sensu Stricto by LightCycler Fluorescence Melting Curve Analysis of a PCR Product of the recA Gene

To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequ...

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Udgivet i:J Clin Microbiol
Principais autores: Pietilä, Johanna, He, Qiushui, Oksi, Jarmo, Viljanen, Matti K.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2000
Fag:
Bacteriology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87021/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10878080/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.7.2756-2759.2000
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