Quantitation of Varicella-Zoster Virus DNA in Whole Blood, Plasma, and Serum by PCR and Electrochemiluminescence

We describe a highly sensitive assay for quantitation of varicella-zoster virus (VZV) DNA in blood, involving PCR amplification, solution hybridization with Tris-(2,2′-bipyridine)-ruthenium(II) chelate-labeled probes, and measurement by electrochemiluminescence (ECL). Extraction and amplification ef...

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Bibliographic Details
Main Authors: de Jong, Menno D., Weel, Jan F. L., Schuurman, Tim, Wertheim-van Dillen, Pauline M. E., Boom, René
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Virology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC86970/
https://ncbi.nlm.nih.gov/pubmed/10878045
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