Detection of and Discrimination between Gram-Positive and Gram-Negative Bacteria in Intraocular Samples by Using Nested PCR

A nested PCR protocol has been developed for the detection of and discrimination between 14 species of gram-positive and -negative bacteria in samples of ocular fluids. First-round PCR with pan-bacterial oligonucleotide primers, based on conserved sequences of the 16S ribosomal gene, was followed by...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Carroll, Nora M., Jaeger, Emma E. M., Choudhury, Sarah, Dunlop, Anthony A. S., Matheson, Melville M., Adamson, Peter, Okhravi, Narciss, Lightman, Susan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Bacteriology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86579/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790093/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.5.1753-1757.2000
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