Detection of and Discrimination between Gram-Positive and Gram-Negative Bacteria in Intraocular Samples by Using Nested PCR

A nested PCR protocol has been developed for the detection of and discrimination between 14 species of gram-positive and -negative bacteria in samples of ocular fluids. First-round PCR with pan-bacterial oligonucleotide primers, based on conserved sequences of the 16S ribosomal gene, was followed by...

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Main Authors: Carroll, Nora M., Jaeger, Emma E. M., Choudhury, Sarah, Dunlop, Anthony A. S., Matheson, Melville M., Adamson, Peter, Okhravi, Narciss, Lightman, Susan
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2000
主題:
Bacteriology
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC86579/
https://ncbi.nlm.nih.gov/pubmed/10790093
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