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Rapid Discrimination of Monkey B Virus from Human Herpes Simplex Viruses by PCR in the Presence of Betaine

A PCR method to amplify DNA segments of the glycoprotein G gene of monkey B virus (BV) was achieved by adding betaine to the PCR mixture, in spite of the high G+C content of this gene. No product was obtained when DNA of human herpes simplex viruses (HSVs) was used as the template under the same con...

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Autors principals: Hirano, Makoto, Nakamura, Shin, Okada, Maki, Ueda, Masahiro, Mukai, Ryozaburo
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC86391/
https://ncbi.nlm.nih.gov/pubmed/10699035
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