Plant Enzymes but Not Agrobacterium VirD2 Mediate T-DNA Ligation In Vitro

Agrobacterium tumefaciens, a gram-negative soil bacterium, transfers DNA to many plant species. In the plant cell, the transferred DNA (T-DNA) is integrated into the genome. An in vitro ligation-integration assay has been designed to investigate the mechanism of T-DNA ligation and the factors involv...

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Bibliographic Details
Main Authors: Ziemienowicz, Alicja, Tinland, Bruno, Bryant, John, Gloeckler, Veronique, Hohn, Barbara
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
DNA Dynamics and Chromosome Structure
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC86106/
https://ncbi.nlm.nih.gov/pubmed/10938108
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