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Comparison of PCR-Restriction Fragment Length Polymorphism Analysis and PCR-Direct Sequencing Methods for Differentiating Helicobacter pylori ureB Gene Variants

A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the Helicobacter pylori genes is widely used to differentiate strains. However, with this typing method only a single base change at a specific restriction site can be detected. In addition, it is unclear whether the nucleotid...

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Published in:J Clin Microbiol
Main Authors: Tanahashi, Toshihito, Kita, Masakazu, Kodama, Tadashi, Sawai, Naoki, Yamaoka, Yoshio, Mitsufuji, Shoji, Katoh, Fumitaka, Imanishi, Jiro
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2000
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86046/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10618081/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.1.165-169.2000
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