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Evaluation of a Nested Reverse Transcription-PCR Assay Based on the Nucleoprotein Gene for Diagnosis of Spontaneous and Experimental Bovine Respiratory Syncytial Virus Infections

The first nested reverse transcription (RT)-PCR based on the nucleoprotein gene (n RT-PCR-N) of the bovine respiratory syncytial virus (BRSV) has been developed and optimized for the detection of BRSV in bronchoalveolar lavage fluid cells of calves. This test is characterized by a low threshold of d...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Principais autores: Valarcher, Jean-François, Bourhy, Hervé, Gelfi, Jacqueline, Schelcher, François
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1999
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84970/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10325337/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.6.1858-1862.1999
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