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Serotyping of Adenoviruses on Conjunctival Scrapings by PCR and Sequence Analysis

To detect and identify adenovirus (Ad), we investigated hypervariable regions (HVRs) of Ad by using a combination of PCR and direct sequencing (PCR-sequence) method. Primers for nested PCR to amplify the conserved region in the hexon protein containing HVRs were designed based on hexon gene sequence...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Takeuchi, Satoshi, Itoh, Norihiko, Uchio, Eiichi, Aoki, Koki, Ohno, Shigeaki
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1999
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84965/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10325334/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.6.1839-1845.1999
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