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Improved Mg(2+)-Based Reverse Transcriptase Assay for Detection of Primate Retroviruses

The reverse transcriptase (RT) assay is a simple, relatively inexpensive, widely used assay that can detect all retroviruses (known and novel retroviruses as well as infectious and defective retroviruses) on the basis of the divalent cation requirement of their RT enzyme, i.e., Mg(2+) or Mn(2+). Des...

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Autors principals: Sears, Johnna F., Repaske, Roy, Khan, Arifa S.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC84929/
https://ncbi.nlm.nih.gov/pubmed/10325311
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