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Simultaneous Detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA Gene-Directed Multiplex PCR

In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 cl...

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Main Authors: Conrads, Georg, Flemmig, Thomas F., Seyfarth, Ilse, Lampert, Friedrich, Lütticken, Rudolf
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1999
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC84855/
https://ncbi.nlm.nih.gov/pubmed/10203541
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