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Identification of Contaminating Fungal DNA Sequences in Zymolyase

When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Principais autores: Rimek, Dagmar, Garg, Amar P., Haas, Walter H., Kappe, Reinhard
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1999
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84574/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9986868/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.3.830-831.1999
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