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Rapid Identification and Typing of Staphylococcus aureus by PCR-Restriction Fragment Length Polymorphism Analysis of the aroA Gene

The Staphylococcus aureus aroA gene, which encodes 5-enolpyruvylshikimate-3-phosphate synthase, was used as a target for the amplification of a 1,153-bp DNA fragment by PCR with a pair of primers of 24 and 19 nucleotides. The PCR products, which were detected by agarose gel electrophoresis, were amp...

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Autors principals: Marcos, Javier Yugueros, Soriano, Alberto Cascón, Salazar, María Sánchez, Moral, Carmen Hernanz, Ramos, Susana Suárez, Smeltzer, Mark S., Carrasco, German Naharro
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC84472/
https://ncbi.nlm.nih.gov/pubmed/9986814
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