Clinical Evaluation of an In-House Reverse Transcription-Competitive PCR for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma

An in-house reverse transcription (RT)-competitive PCR (RT-cPCR) for the quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma samples was developed and validated. The procedure involves (i) extraction of RNA with spin columns, (ii) ready-to-use bead-mediated RT, (iii) competitiv...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Zazzi, Maurizio, Romano, Laura, Catucci, Marinunzia, Venturi, Giulietta, De Milito, Angelo, Valensin, Pier E.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1999
Schlagworte:	Virology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84299/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9889213/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.2.333-338.1999
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