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A Quantitative Heterokaryon Assay to Measure the Nucleocytoplasmic Shuttling of Proteins

Many proteins appear exclusively nuclear at steady-state but in fact shuttle continuously back and forth between the nucleus and the cytoplasm. For example, nuclear RNA-binding proteins (RBPs) often accompany mRNAs to the cytoplasm, where they can regulate subcellular localization, translation and/o...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: McNicoll, François, Müller-McNicoll, Michaela
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8328641/
https://ncbi.nlm.nih.gov/pubmed/34395784
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2472
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