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Protocol for tissue clearing and 3D analysis of dopamine neurons in the developing mouse midbrain
Advances in tissue clearing enable analysis of complex migratory patterns of developing neurons in whole intact tissue. Here, we implemented a modified version of 3DISCO to study migration of midbrain dopamine (DA) neurons. We provide a detailed protocol starting from whole-brain immunostaining, tis...
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| Udgivet i: | STAR Protoc |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Elsevier
2021
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8326876/ https://ncbi.nlm.nih.gov/pubmed/34377993 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100669 |
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