Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli under the control of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations

We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid pBAD/3C/bTGase, a bicistronic expression system, in bioreactor cultivation. Batch and fed-batch controlled as DO-stat strategies were employed for the production o...

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Detalhes bibliográficos
Publicado no:Braz J Microbiol
Main Authors: Duarte, Lovaine Silva, Matte, Carla Roberta, Dall Cortivo, Paulo Roberto, Nunes, José Eduardo Sacconi, Barsé, Laisa Quadros, Bizarro, Cristiano Valim, Ayub, Marco Antônio Záchia
Formato: Artigo
Idioma:Inglês
Publicado em: Springer International Publishing 2021
Assuntos:
Biotechnology and Industrial Microbiology - Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8324676/
https://ncbi.nlm.nih.gov/pubmed/34008152
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s42770-021-00521-3
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