Quantification of long non-coding RNAs using qRT-PCR: comparison of different cDNA synthesis methods and RNA stability

INTRODUCTION: Long non-coding RNAs (lncRNAs), a class of regulatory RNA molecules, are over 200 nucleotides long and could be used as a new potential biomarker, but their detection methods such as qRT-PCR are still not validated, and the influence of RNA degradation on lncRNA quantification is not c...

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Vydáno v:Arch Med Sci
Hlavní autoři: Kolenda, Tomasz, Ryś, Marcel, Guglas, Kacper, Teresiak, Anna, Bliźniak, Renata, Mackiewicz, Jacek, Lamperska, Katarzyna
Médium: Artigo
Jazyk:Inglês
Vydáno: Termedia Publishing House 2019
Témata:
Basic Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8314425/
https://ncbi.nlm.nih.gov/pubmed/34336028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5114/aoms.2019.82639
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