Functional assays with human patient-derived enteroid monolayers to assess the human gut barrier

Here, we describe the use of polarized patient enteroid-derived monolayers (EDMs) to assess the impact of e-cigarettes on the human gut barrier. These EDMs can be adapted to culture in a 96-well plate for high-throughput screening. We model the effect of e-cigarettes by combining pathogens, enteroid...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Sayed, Ibrahim M., Tindle, Courtney, Fonseca, Ayden G., Ghosh, Pradipta, Das, Soumita
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8313751/
https://ncbi.nlm.nih.gov/pubmed/34337445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100680
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