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A multi‐functional genetic manipulation system and its use in high‐level expression of a β‐mannanase mutant with high specific activity in Pichia pastoris
To further extend the practical application of a thermostable and acidic resistance β‐mannanase (ManAK) in animal feed additives, an effective strategy that combined directed evolution and metabolic engineering was developed. Four positive mutants (P191M, P194E, S199G and S268Q) with enhanced specif...
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| Publicado no: | Microb Biotechnol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8313266/ https://ncbi.nlm.nih.gov/pubmed/33942496 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13812 |
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