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Tracking single particles for hours via continuous DNA-mediated fluorophore exchange

Monitoring biomolecules in single-particle tracking experiments is typically achieved by employing fixed organic dyes or fluorescent fusion proteins linked to a target of interest. However, photobleaching typically limits observation times to merely a few seconds, restricting downstream statistical...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Stehr, Florian, Stein, Johannes, Bauer, Julian, Niederauer, Christian, Jungmann, Ralf, Ganzinger, Kristina, Schwille, Petra
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8295357/
https://ncbi.nlm.nih.gov/pubmed/34290254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-021-24223-4
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