Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.)

Difficulty in protoplast regeneration is a major obstacle to apply the CRISPR/Cas9 gene editing technique effectively in research and breeding of rapeseed (Brassica napus L.). The present study describes for the first time a rapid and efficient protocol for the isolation, regeneration and transfecti...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Li, Xueyuan, Sandgrind, Sjur, Moss, Oliver, Guan, Rui, Ivarson, Emelie, Wang, Eu Sheng, Kanagarajan, Selvaraju, Zhu, Li-Hua
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2021
Assuntos:
Plant Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8294089/
https://ncbi.nlm.nih.gov/pubmed/34305978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2021.680859
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