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Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM)

The data quality of high-resolution imaging can be markedly improved with active stabilization, which is based on feedback loops within the microscope that maintain the sample in the same location throughout the experiment. The purpose is to provide a highly accurate focus lock, therefore eliminatin...

Description complète

Enregistré dans:
Détails bibliographiques
Publié dans:Bio Protoc
Auteurs principaux: Coelho, Simao, Baek, Jongho, Gooding, J. Justin, Gaus, Katharina
Format: Artigo
Langue:Inglês
Publié: Bio-Protocol 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8292130/
https://ncbi.nlm.nih.gov/pubmed/34327271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.4074
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