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Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM)

The data quality of high-resolution imaging can be markedly improved with active stabilization, which is based on feedback loops within the microscope that maintain the sample in the same location throughout the experiment. The purpose is to provide a highly accurate focus lock, therefore eliminatin...

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Bibliografski detalji
Izdano u:Bio Protoc
Glavni autori: Coelho, Simao, Baek, Jongho, Gooding, J. Justin, Gaus, Katharina
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2021
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8292130/
https://ncbi.nlm.nih.gov/pubmed/34327271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.4074
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